An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN.

Loewenberg M, Verhaar AP, Bilderbeek J, Marle J, Buttgereit F, Peppelenbosch MP, van Deventer SJ, Hommes DW

Although glucocorticoid (GC)-induced nongenomic effects have been reported, the underlying mechanisms remain unexplained. We previously described that lymphocyte-specific protein tyrosine kinase (LCK) and FYN oncogene related to SRC, FGR, YES (FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Here we characterize the underlying molecular mechanism. The present study shows that the GC receptor is part of a TCR-linked multiprotein complex ... [more]

EMBO Rep. Oct. 01, 2006; 7(10);1023-9 [Pubmed: 16888650]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ZAP70

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [NAS]non-membrane spanning protein tyrosine kinase activity [IBA, TAS]protein binding [IPI]protein tyrosine kinase activity [IDA, NAS, TAS]receptor binding [IBA]

Gene Ontology Cellular Component

HSPA4