Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Pdlim2, a novel PDZ-LIM domain protein, interacts with alpha-actinins and filamin A.

Torrado M, Senatorov VV, Trivedi R, Fariss RN, Tomarev SI

To characterize properties of Pdlim2, a novel PDZ and LIM domain-containing protein.cDNA encoding Pdlim2 was identified in a cDNA library of transcripts expressed in the tissues of the rat eye irido-corneal angle. The expression pattern of the Pdlim2 gene was studied by Northern blot analysis and in situ hybridization. Proteins interacting with Pdlim2 were identified by pull-down assay and mass ... [more]

Invest. Ophthalmol. Vis. Sci. Nov. 01, 2004; 45(11);3955-63 [Pubmed: 15505042]

Throughput

  • Low Throughput

Additional Notes

  • pulldown followed by MS for identification

Curated By

  • BioGRID