Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Pdlim2, a novel PDZ-LIM domain protein, interacts with alpha-actinins and filamin A.

Torrado M, Senatorov VV, Trivedi R, Fariss RN, Tomarev SI

To characterize properties of Pdlim2, a novel PDZ and LIM domain-containing protein.cDNA encoding Pdlim2 was identified in a cDNA library of transcripts expressed in the tissues of the rat eye irido-corneal angle. The expression pattern of the Pdlim2 gene was studied by Northern blot analysis and in situ hybridization. Proteins interacting with Pdlim2 were identified by pull-down assay and mass ... [more]

Invest. Ophthalmol. Vis. Sci. Nov. 01, 2004; 45(11);3955-63 [Pubmed: 15505042]

Throughput

  • Low Throughput

Additional Notes

  • pulldown followed by MS for identification

Curated By

  • BioGRID