PDLIM2
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MYH9
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- actin cytoskeleton reorganization [ISS]
- actin filament-based movement [ISS]
- angiogenesis [ISS]
- blood vessel endothelial cell migration [ISS]
- membrane protein ectodomain proteolysis [ISS]
- monocyte differentiation [ISS]
- platelet formation [ISS]
- positive regulation of calcium ion transport into cytosol [IMP]
- positive regulation of endocytosis [IMP]
- positive regulation of phagocytosis [IMP]
- protein transport [ISS]
- regulation of cell shape [ISS]
- sodium ion transmembrane transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actomyosin contractile ring [ISS]
- cell leading edge [ISS]
- cleavage furrow [ISS]
- cytoplasm [IDA, ISS]
- cytosol [ISS]
- endosome [IDA]
- integrin complex [ISS]
- lateral plasma membrane [IDA]
- nucleus [ISS]
- phagocytic vesicle [IDA]
- plasma membrane [ISS]
- protein complex [IDA, ISS]
- ruffle [ISS]
- stress fiber [ISS]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Pdlim2, a novel PDZ-LIM domain protein, interacts with alpha-actinins and filamin A.
To characterize properties of Pdlim2, a novel PDZ and LIM domain-containing protein.cDNA encoding Pdlim2 was identified in a cDNA library of transcripts expressed in the tissues of the rat eye irido-corneal angle. The expression pattern of the Pdlim2 gene was studied by Northern blot analysis and in situ hybridization. Proteins interacting with Pdlim2 were identified by pull-down assay and mass ... [more]
Throughput
- Low Throughput
Additional Notes
- pulldown followed by MS for identification
Curated By
- BioGRID