BAIT

PRKCB

Pkcb, Prkcb1
protein kinase C, beta
GO Process (25)
GO Function (10)
GO Component (8)
Rattus norvegicus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Molecular cloning and characterization of a novel protein kinase C-interacting protein with structural motifs related to RBCC family proteins.

Tokunaga C, Kuroda S, Tatematsu K, Nakagawa N, Ono Y, Kikkawa U

A novel protein kinase C (PKC)-interacting protein was identified by the yeast two-hybrid screening using the regulatory domain of PKC beta I as a bait. The protein contained several structural motifs such as two putative coiled-coil regions, a RING-finger, a B-box, and a B-box-like motif in the order from NH2- to COOH-terminals. The molecular organization of the protein resembles the ... [more]

Biochem. Biophys. Res. Commun. Mar. 17, 1998; 244(2);353-9 [Pubmed: 9514928]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RBCK1 PRKCB
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID