UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPARA
Gene Ontology Biological Process
- behavioral response to nicotine [IDA]
- circadian regulation of gene expression [ISO, ISS]
- enamel mineralization [ISO]
- epidermis development [ISO]
- fatty acid metabolic process [IMP, ISO]
- heart development [IEP]
- intracellular receptor signaling pathway [IMP, ISO]
- lipoprotein metabolic process [IMP]
- negative regulation of appetite [ISO, ISS]
- negative regulation of blood pressure [IMP]
- negative regulation of cholesterol storage [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- negative regulation of neuron death [IDA, IMP]
- negative regulation of protein binding [IMP]
- negative regulation of receptor biosynthetic process [ISO]
- negative regulation of sequestering of triglyceride [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription regulatory region DNA binding [IMP]
- positive regulation of fatty acid oxidation [ISO]
- positive regulation of gluconeogenesis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO, ISS]
- regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of circadian rhythm [ISO, ISS]
- regulation of fatty acid metabolic process [ISO, TAS]
- regulation of gene expression [ISO]
- regulation of glycolytic by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA]
- response to hypoxia [IDA]
- response to insulin [IMP]
- transcription from RNA polymerase II promoter [ISO]
- wound healing [ISO]
Gene Ontology Molecular Function- DNA binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- drug binding [ISO, ISS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IMP, ISO]
- lipid binding [ISO, ISS]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein complex binding [IMP, IPI]
- protein domain specific binding [IDA]
- receptor activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
- DNA binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- drug binding [ISO, ISS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IMP, ISO]
- lipid binding [ISO, ISS]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein complex binding [IMP, IPI]
- protein domain specific binding [IDA]
- receptor activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Regulation of peroxisome proliferator-activated receptor-alpha by MDM2.
Peroxisome proliferator-activated receptor-alpha (PPARalpha) belongs to the nuclear receptor (NR) family of transcription factors and regulates lipid and glucose metabolism. Like other NRs, the regulation of gene expression by PPARalpha depends on cofactor recruitment to the transcription complex and multiple protein-protein interactions. In this study, Murine Double Minute 2 (MDM2), an E3 ubiquitin ligase, is identified as a PPARalpha-interacting protein ... [more]
Throughput
- Low Throughput
Additional Notes
- likely ubiquitin conjugate
- source of ubiquitin not clear
Curated By
- BioGRID