STXBP1
Gene Ontology Biological Process
- axon target recognition [ISO]
- exocytosis [ISO]
- long term synaptic depression [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of synaptic transmission, GABAergic [ISO]
- neuromuscular synaptic transmission [ISO]
- neurotransmitter secretion [ISO]
- platelet aggregation [ISO]
- platelet degranulation [ISO]
- positive regulation of calcium ion-dependent exocytosis [ISO]
- positive regulation of exocytosis [IMP]
- protein localization to plasma membrane [ISO]
- protein stabilization [IDA]
- regulation of synaptic vesicle priming [IMP]
- synaptic vesicle maturation [ISO]
- vesicle docking involved in exocytosis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
STX3
Gene Ontology Biological Process
- exocytosis [ISO]
- intracellular protein transport [IBA]
- intracellular transport [TAS]
- long-term synaptic potentiation [ISO]
- membrane fusion [IDA]
- neuron projection development [IDA]
- positive regulation of cell adhesion [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of chemotaxis [ISO]
- positive regulation of protein localization to cell surface [ISO]
- positive regulation of protein localization to plasma membrane [ISO]
- synaptic vesicle docking [TAS]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA]
- apical plasma membrane [ISO]
- azurophil granule [ISO]
- cell [ISO]
- cell-cell junction [ISO]
- dendrite [ISO]
- endomembrane system [IBA]
- extracellular vesicular exosome [ISO]
- growth cone [IDA]
- integral component of membrane [IBA]
- lamellipodium [ISO]
- membrane [ISO]
- neuron projection [IDA]
- plasma membrane [IDA, ISO, TAS]
- secretory granule [ISO]
- specific granule [ISO]
- synaptic vesicle [IBA]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A novel ubiquitous form of Munc-18 interacts with multiple syntaxins. Use of the yeast two-hybrid system to study interactions between proteins involved in membrane traffic.
Munc-18-1 is a 67-kDa neuronal protein that binds tightly to syntaxin 1 and functions in synaptic vesicle exocytosis (Hata, Y., Slaughter, C.A., and Suedhof, T.C. (1993a) Nature 366, 347-351). We have now characterized a new Munc-18 isoform, Munc-18-2, that exhibits 63% amino acid sequence identity with Munc-18-1. Munc-18-2 is expressed in most tissues, whereas Munc-18-1 is primarily expressed in brain. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX3 STXBP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| STX3 STXBP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| STX3 STXBP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| STX3 STXBP1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID