Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The human Ino80 binds to microtubule via the E-hook of tubulin: implications for the role in spindle assembly.

Park EJ, Hur SK, Lee HS, Lee SA, Kwon J

The human INO80 chromatin remodeling complex, comprising the Ino80 ATPase (hIno80) and the associated proteins such as Tip49a, has been implicated in a variety of nuclear processes other than transcription. We previously have found that hIno80 interacts with tubulin and co-localizes with the mitotic spindle and is required for spindle formation. To better understand the role of hIno80 in spindle ... [more]

Biochem. Biophys. Res. Commun. Dec. 16, 2011; 416(3);416-20 [Pubmed: 22133677]

Throughput

  • Low Throughput

Curated By

  • BioGRID