BAIT

BANF1

BAF, BCRP1, D14S1460, NGPS
barrier to autointegration factor 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Barrier-to-Autointegration Factor influences specific histone modifications.

Montes de Oca R, Andreassen PR, Wilson KL

Defects in the nuclear envelope or nuclear 'lamina' networks cause disease and can perturb histone posttranslational (epigenetic) regulation. Barrier-to-Autointegration Factor (BAF) is an essential but enigmatic lamina component that binds lamins, LEM-domain proteins, DNA and histone H3 directly. We report that BAF copurified with nuclease-digested mononucleosomes and associated with modified histones in vivo. BAF overexpression significantly reduced global histone H3 ... [more]

Nucleus Nov. 01, 2011; 2(6);580-90 [Pubmed: 22127260]

Throughput

  • Low Throughput

Curated By

  • BioGRID