A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

A single amino acid substitution in a IIIF bHLH transcription factor AtMYC1 leads to trichome and root hair patterning defects by abolishing its interaction with partner proteins in Arabidopsis.

Zhao H, Wang X, Zhu D, Cui S, Li X, Cao Y, Ma L

Plant trichomes and root hairs are powerful models for the study of cell fate determination. In Arabidopsis thaliana, trichome and root hair initiation requires a combination of three groups of proteins, including the WD40 repeat protein TRANSPARENT TESTA GLABRA1 (TTG1), R2R3 repeat MYB protein GLABRA1 (GL1) or WEREWOLF (WER), and the IIIf subfamily of basic helix-loop-helix (bHLH) protein GLABRA3 (GL3) ... [more]

Unknown Feb. 14, 2012; 0(0); [Pubmed: 22334670]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TRY ATMYC1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zhao H (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TRY

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

ATMYC1