Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Proteasome subunit Rpn13 is a novel ubiquitin receptor.

Husnjak K, Elsasser S, Zhang N, Chen X, Randles L, Shi Y, Hofmann K, Walters KJ, Finley D, Dikic I

Proteasomal receptors that recognize ubiquitin chains attached to substrates are key mediators of selective protein degradation in eukaryotes. Here we report the identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome. Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain, which binds K48-linked diubiquitin with an affinity of approximately ... [more]

Nature May. 22, 2008; 453(7194);481-8 [Pubmed: 18497817]

Throughput

Low Throughput

Additional Notes

#LPPI
Likely protein-protein interaction

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ADRM1 UBC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Husnjak K (2008)	Low	-	BioGRID	617471
UBC ADRM1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhang X (2017)	High	-	BioGRID	2297284

Curated By

BioGRID

Interaction Summary

ADRM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

endopeptidase activator activity [ISO]protease binding [ISO]proteasome binding [ISO]

Gene Ontology Cellular Component

UBC

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [ISO]protease binding [ISO]protein binding [IPI]