RELA
Gene Ontology Biological Process
- NIK/NF-kappaB signaling [IBA]
- cellular response to hydrogen peroxide [ISO]
- cellular response to interleukin-1 [ISO]
- cellular response to interleukin-6 [ISO]
- cellular response to nicotine [ISO]
- cellular response to peptide hormone stimulus [ISO]
- cellular response to stress [IBA]
- cellular response to tumor necrosis factor [ISO]
- cytokine-mediated signaling pathway [ISO]
- defense response [IMP]
- hair follicle development [IMP]
- inflammatory response [ISO]
- innate immune response [IBA]
- liver development [IMP]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of extrinsic apoptotic signaling pathway [IDA, ISO]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IBA, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- nucleotide-binding oligomerization domain containing 2 signaling pathway [ISO]
- organ morphogenesis [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA, ISO]
- positive regulation of Schwann cell differentiation [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of chondrocyte differentiation [ISO]
- positive regulation of interleukin-12 biosynthetic process [IDA]
- positive regulation of miRNA metabolic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IBA, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO, TAS]
- regulation of inflammatory response [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA]
- response to UV-B [ISO]
- response to bacterium [IDA]
- response to cytokine [IBA]
- response to interleukin-1 [ISO]
- response to muramyl dipeptide [IDA]
- response to muscle stretch [IDA]
- response to organic substance [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- activating transcription factor binding [ISO]
- ankyrin repeat binding [IPI]
- chromatin binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- phosphate ion binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [IPI, ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
- DNA binding [IDA, ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- activating transcription factor binding [ISO]
- ankyrin repeat binding [IPI]
- chromatin binding [IDA, ISO]
- enzyme binding [IPI]
- identical protein binding [ISO]
- phosphate ion binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [IPI, ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
NFKBIA
Gene Ontology Biological Process
- cytoplasmic sequestering of NF-kappaB [ISO]
- cytoplasmic sequestering of transcription factor [ISO]
- lipopolysaccharide-mediated signaling pathway [IDA]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of Notch signaling pathway [IMP]
- negative regulation of lipid storage [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- negative regulation of myeloid cell differentiation [IMP]
- nucleotide-binding oligomerization domain containing 1 signaling pathway [IDA]
- nucleotide-binding oligomerization domain containing 2 signaling pathway [IDA]
- positive regulation of cellular protein metabolic process [ISO]
- positive regulation of cholesterol efflux [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IMP]
- protein import into nucleus, translocation [IDA]
- regulation of cell proliferation [IDA]
- regulation of gene expression [IMP]
- response to exogenous dsRNA [IDA]
- response to lipopolysaccharide [IDA]
- response to muramyl dipeptide [IDA]
- response to muscle stretch [IDA]
- toll-like receptor 4 signaling pathway [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Suppression of p65 phosphorylation coincides with inhibition of IkappaBalpha polyubiquitination and degradation.
Transcription factor nuclear factor-kappaB (NF-kappaB) is held in the cytoplasm in an inactive state by IkappaB inhibitors. Oncogenic activation of NF-kappaB is achieved by stimulus-induced ubiquitination and subsequent proteasome-mediated degradation of IkappaBalpha. Once released from the inhibitor, NF-kappaB/p65 enters the nucleus. A pre-requisite for cytokine-induced IkappaBalpha ubiquitination and degradation is the phosphorylation of IkappaBalpha at S32/S36. Phosphorylation of IkappaBalpha alone, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NFKBIA RELA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NFKBIA RELA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RELA NFKBIA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RELA NFKBIA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 591059 |
Curated By
- BioGRID