BAIT

MCM5

CG4082, DmCDC46, DmCDC465, DmMCM5, DmeMCM5, Dmel\CG4082, PCR4, Dmel_CG4082
Minichromosome maintenance 5
Drosophila melanogaster
PREY

CG18745

Dmel\CG18745, Dmel_CG18745
CG18745 gene product from transcript CG18745-RA
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A Drosophila protein-interaction map centered on cell-cycle regulators.

Stanyon CA, Liu G, Mangiola BA, Patel N, Giot L, Kuang B, Zhang H, Zhong J, Finley RL

BACKGROUND: Maps depicting binary interactions between proteins can be powerful starting points for understanding biological systems. A proven technology for generating such maps is high-throughput yeast two-hybrid screening. In the most extensive screen to date, a Gal4-based two-hybrid system was used recently to detect over 20,000 interactions among Drosophila proteins. Although these data are a valuable resource for insights into ... [more]

Genome Biol. Dec. 04, 2004; 5(12);R96 [Pubmed: 15575970]

Throughput

  • High Throughput

Curated By

  • BioGRID