An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Epigenetic modulation of the renal Î²-adrenergic-WNK4 pathway in salt-sensitive hypertension.

Mu S, Shimosawa T, Ogura S, Wang H, Uetake Y, Kawakami-Mori F, Marumo T, Yatomi Y, Geller DS, Tanaka H, Fujita T

How high salt intake increases blood pressure is a key question in the study of hypertension. Salt intake induces increased renal sympathetic activity resulting in sodium retention. However, the mechanisms underlying the sympathetic control of renal sodium excretion remain unclear. In this study, we found that Î²(2)-adrenergic receptor (Î²(2)AR) stimulation led to decreased transcription of the gene encoding WNK4, a ... [more]

Nat. Med. May. 01, 2011; 17(5);573-80 [Pubmed: 21499270]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HDAC8

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase activity [TAS]transcription factor binding [TAS]

Gene Ontology Cellular Component

HIST3H3