BAIT

NDRG1

CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
N-myc downstream regulated 1
Homo sapiens
PREY

UPF1

HUPF1, NORF1, RENT1, pNORF1, smg-2
UPF1 regulator of nonsense transcripts homolog (yeast)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells.

Tu LC, Yan X, Hood L, Lin B

NDRG1 is known to play important roles in both androgen-induced cell differentiation and inhibition of prostate cancer metastasis. However, the proteins associated with NDRG1 function are not fully enumerated. Using coimmunoprecipitation and mass spectrometry analysis, we identified 58 proteins that interact with NDRG1 in prostate cancer cells. These proteins include nuclear proteins, adhesion molecules, endoplasmic reticulum (ER) chaperons, proteasome subunits, ... [more]

Mol. Cell Proteomics Apr. 01, 2007; 6(4);575-88 [Pubmed: 17220478]

Throughput

  • High Throughput

Ontology Terms

  • cell line: lncap cell (BTO:0001321)
  • disease: prostate carcinoma (DOID:10286)
  • chemical: 17beta-hydroxy-17-methylestra-4,9,11-trien-3-one (CHEBI:379896)

Curated By

  • BioGRID