Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Fibroblast growth factor 3, a protein with a dual subcellular fate, is interacting with human ribosomal protein S2.

Antoine M, Reimers K, Wirz W, Gressner AM, Mueller R, Kiefer P

The secreted isoform of fibroblast growth factor 3 (FGF3) induces a mitogenic cell response, while the nuclear form inhibits cell proliferation. Recently, we identified a nucleolar FGF3-binding protein which is implicated in processing of pre-rRNA as a possible target of nuclear FGF3 signalling. Here, we report a second candidate protein identified by a yeast two-hybrid screen for nuclear FGF3 action, ... [more]

Biochem. Biophys. Res. Commun. Dec. 16, 2005; 338(2);1248-55 [Pubmed: 16263090]

Throughput

  • Low Throughput

Curated By

  • BioGRID