BAIT
UBC
HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- DNA repair [TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- I-kappaB kinase/NF-kappaB signaling [TAS]
- JNK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- Notch receptor processing [TAS]
- Notch signaling pathway [TAS]
- RNA metabolic process [TAS]
- T cell receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- carbohydrate metabolic process [TAS]
- cellular response to hypoxia [TAS]
- cytokine-mediated signaling pathway [TAS]
- endosomal transport [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- glucose metabolic process [TAS]
- glycogen biosynthetic process [TAS]
- innate immune response [TAS]
- intracellular transport of virus [TAS]
- ion transmembrane transport [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- negative regulation of type I interferon production [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [TAS]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- positive regulation of type I interferon production [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- regulation of apoptotic process [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of type I interferon production [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- transmembrane transport [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral protein processing [TAS]
- virion assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ESR1
ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1
estrogen receptor 1 (alpha)
GO Process (46)
GO Function (19)
GO Component (12)
Gene Ontology Biological Process
- Sertoli cell proliferation [ISO]
- androgen metabolic process [IMP]
- antral ovarian follicle growth [IMP]
- cell growth [NAS]
- cellular response to estradiol stimulus [ISO]
- cellular response to estrogen stimulus [IMP]
- epithelial cell development [IMP]
- epithelial cell proliferation involved in mammary gland duct elongation [IMP]
- intracellular estrogen receptor signaling pathway [ISO]
- intracellular receptor signaling pathway [IDA]
- intracellular steroid hormone receptor signaling pathway [ISO]
- male gonad development [IMP]
- mammary gland alveolus development [IMP]
- mammary gland branching involved in pregnancy [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of glucose import [ISO]
- negative regulation of mitosis [NAS]
- negative regulation of neuron death [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of smooth muscle cell proliferation [ISO]
- negative regulation of triglyceride metabolic process [ISO]
- phospholipase C-activating G-protein coupled receptor signaling pathway [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of epidermal growth factor receptor signaling pathway [ISO]
- positive regulation of fibroblast proliferation [IMP]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of phospholipase C activity [ISO]
- positive regulation of retinoic acid receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis [IMP]
- prostate epithelial cord elongation [IMP]
- regulation of apoptotic process [IMP]
- regulation of branching involved in prostate gland morphogenesis [IMP]
- regulation of neuron apoptotic process [ISO]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA]
- response to estradiol [ISO]
- response to estrogen [ISO]
- steroid hormone mediated signaling pathway [ISO]
- transcription from RNA polymerase II promoter [IDA]
- uterus development [IGI]
- vagina development [IGI]
Gene Ontology Molecular Function- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [ISO]
- chromatin binding [IDA, ISO]
- core promoter sequence-specific DNA binding [ISO]
- enzyme binding [ISO]
- estrogen receptor activity [ISO]
- estrogen response element binding [ISO]
- estrogen-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- hormone binding [ISO]
- identical protein binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein complex binding [ISO]
- steroid binding [ISO]
- steroid hormone receptor activity [ISO]
- transcription factor binding [IPI, ISO]
- type 1 metabotropic glutamate receptor binding [ISO]
- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [ISO]
- chromatin binding [IDA, ISO]
- core promoter sequence-specific DNA binding [ISO]
- enzyme binding [ISO]
- estrogen receptor activity [ISO]
- estrogen response element binding [ISO]
- estrogen-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- hormone binding [ISO]
- identical protein binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein complex binding [ISO]
- steroid binding [ISO]
- steroid hormone receptor activity [ISO]
- transcription factor binding [IPI, ISO]
- type 1 metabotropic glutamate receptor binding [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Cross talk in hormonally regulated gene transcription through induction of estrogen receptor ubiquitylation.
Estrogen tightly regulates the levels of circulating gonadotropins, but a direct effect of estrogen receptor alpha (ERalpha) on the mammalian LHbeta gene has remained poorly defined. We demonstrate here that ERalpha can associate with the LHbeta promoter through interactions with Sf-1 and Pitx1 without requiring an estrogen response element (ERE). We show that gonadotropin-releasing hormone (GnRH) promotes ERalpha ubiquitylation and ... [more]
Mol. Cell. Biol. Aug. 01, 2005; 25(16);7386-98 [Pubmed: 16055746]
Throughput
- Low Throughput
Curated By
- BioGRID