BAIT
MAK10
NAA35, L000000983, YEL053C
Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; expression is glucose-repressible
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CDC34
DNA6, UBC3, SCF E2 ubiquitin-protein ligase catalytic subunit CDC34, L000000271, YDR054C
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [IGI]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein autoubiquitination [IDA, IMP]
- protein polyubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex.
Although many eukaryotic proteins are amino (N)-terminally acetylated, structural mechanisms by which N-terminal acetylation mediates protein interactions are largely unknown. Here, we found that N-terminal acetylation of the E2 enzyme, Ubc12, dictates distinctive E3-dependent ligation of the ubiquitin-like protein Nedd8 to Cul1. Structural, biochemical, biophysical, and genetic analyses revealed how complete burial of Ubc12's N-acetyl-methionine in a hydrophobic pocket in ... [more]
Science Nov. 04, 2011; 334(6056);674-8 [Pubmed: 21940857]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: heat sensitivity (APO:0000147)
Curated By
- BioGRID