KCNJ1
Gene Ontology Biological Process
- cardiovascular system development [ISO]
- cellular response to magnesium ion [IDA]
- kidney development [ISO]
- negative regulation of apoptotic process [IMP]
- post-embryonic development [ISO]
- potassium ion import [IBA]
- potassium ion transport [IMP]
- regulation of G-protein activated inward rectifier potassium channel activity [ISO]
- regulation of ion transmembrane transport [IBA]
- renal sodium ion absorption [ISO]
- response to potassium ion [IEP]
- tissue homeostasis [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
ROMK1 channel activity is regulated by monoubiquitination.
The ubiquitination of proteins can signal their degradation, modify their activity or target them to specific membranes or cellular organelles. Here, we show that monoubiquitination regulates the plasma membrane abundance and function of the potassium channel, ROMK. Immunoprecipitation of proteins obtained from renal cortex and outer medulla with ROMK antibody revealed that this channel was monoubiquitinated. To determine the ubiquitin ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID