BAIT

FUB1

YCR076C

Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene

GO Process (0)

GO Function (1)

GO Component (0)

Gene Ontology Molecular Function

proteasome binding [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

PRE7

PRS3, proteasome core particle subunit beta 6, L000002699, YBL041W

Beta 6 subunit of the 20S proteasome

UPS Project

GO Process (2)

GO Function (0)

GO Component (4)

Gene Ontology Biological Process

proteasomal ubiquitin-independent protein catabolic process [IDA]

proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]

Gene Ontology Cellular Component

endoplasmic reticulum membrane [IC]

nucleus [IC]

proteasome core complex, beta-subunit complex [IDA]

proteasome storage granule [IC]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex.

Hatanaka A, Chen B, Sun JQ, Mano Y, Funakoshi M, Kobayashi H, Ju Y, Mizutani T, Shinmyozu K, Nakayama J, Miyamoto K, Uchida H, Oki M

Silenced chromatin domains are restricted to specific regions. Eukaryotic chromosomes are organized into discrete domains delimited by domain boundaries. From approximately 6,000 genes in Saccharomyces cerevisiae, we previously isolated 55 boundary genes. In this study, we focus on the molecular function of one of boundary genes, YCR076C/FUB1 (function of boundary), whose function has not been clearly defined in vivo. Biochemical ... [more]

Genes Genet. Syst. Mar. 01, 2012; 86(5);305-14 [Pubmed: 22362029]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRE7 FUB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3615761
PRE7 FUB1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.6424	BioGRID	1958823

Curated By

BioGRID

Interaction Summary