asf1, SPCC663.05c
histone chaperone Cia1
Schizosaccharomyces pombe (972h)


SPBC4C3.11, SPBC1778.10c
serine/threonine protein kinase Ppk21
GO Process (3)
GO Function (0)
GO Component (5)
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.


Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast.

Tanae K, Horiuchi T, Matsuo Y, Katayama S, Kawamukai M

The histone H3-H4 chaperone Asf1 is involved in chromatin assembly (or disassembly), histone exchange, regulation of transcription, and chromatin silencing in several organisms. To investigate the essential functions of Asf1 in Schizosaccharomyces pombe, asf1-ts mutants were constructed by random mutagenesis using PCR. One mutant (asf1-33(ts)) was mated with mutants in 77 different kinase genes to identify synthetic lethal combinations. The ... [more]

PLoS ONE Feb. 01, 2012; 7(1);e30472 [Pubmed: 22291963]


  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID