BAIT
CIA1
asf1, SPCC663.05c
histone chaperone Cia1
GO Process (8)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
CHK1
rad27, SPCC1259.13
Chk1 protein kinase
GO Process (5)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- mitotic DNA damage checkpoint [IGI]
- mitotic G2 DNA damage checkpoint [IMP]
- negative regulation of transcription from RNA polymerase II promoter by transcription factor localization involved in response to DNA damage checkpoint signaling [IMP]
- peptidyl-serine phosphorylation [IDA]
- signal transduction involved in DNA damage checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast.
The histone H3-H4 chaperone Asf1 is involved in chromatin assembly (or disassembly), histone exchange, regulation of transcription, and chromatin silencing in several organisms. To investigate the essential functions of Asf1 in Schizosaccharomyces pombe, asf1-ts mutants were constructed by random mutagenesis using PCR. One mutant (asf1-33(ts)) was mated with mutants in 77 different kinase genes to identify synthetic lethal combinations. The ... [more]
PLoS ONE Feb. 01, 2012; 7(1);e30472 [Pubmed: 22291963]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID