A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.

Buttrick GJ, Meadows JC, Lancaster TC, Vanoosthuyse V, Shepperd LA, Hoe KL, Kim DU, Park HO, Hardwick KG, Millar JB

Type 1 phosphatase (PP1) antagonizes Aurora B kinase to stabilize kinetochore-microtubule attachments and to silence the spindle checkpoint. We screened for factors that exacerbate the growth defect of Î”dis2 cells, which lack one of two catalytic subunits of PP1 in fission yeast, and identified Nsk1, a novel protein required for accurate chromosome segregation. During interphase, Nsk1 resides in the nucleolus ... [more]

Mol. Biol. Cell Dec. 01, 2011; 22(23);4486-502 [Pubmed: 21965289]

Throughput

High Throughput

Ontology Terms

vegetative growth (APO:0000106)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FFT3 DIS2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lee J (2017)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DIS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphoprotein phosphatase activity [IDA]protein serine/threonine phosphatase activity [IDA]

Gene Ontology Cellular Component

FFT3

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-dependent ATPase activity [IDA]protein binding [IPI]