BAIT

GSH1

glutamate--cysteine ligase, L000000735, YJL101C
Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ATF2

L000003345, YGR177C
Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The critical role of glutathione in maintenance of the mitochondrial genome.

Ayer A, Tan SX, Grant CM, Meyer AJ, Dawes IW, Perrone GG

Glutathione (GSH) is a key redox buffer and protectant. Growth (approx. one or two divisions) of cells lacking γ-glutamylcysteine synthetase (gsh1) in the absence of GSH led to irreversible respiratory incompetency in all cells, and after five divisions 75% of cells completely lacked mitochondrial DNA (mtDNA). The level of GSH required to allow continuous growth was distinct from that required ... [more]

Free Radic. Biol. Med. Dec. 15, 2010; 49(12);1956-68 [Pubmed: 20888410]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: respiratory metabolism (APO:0000102)

Additional Notes

  • deletion of Atf1 and Atf2 additively decreases the rate of formation of respiratory incompetent cells seen upon deletion of Gsh1
  • deletion of Atf1 decreases the rate of formation of respiratory incompetent cells seen upon deletion of Gsh1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATF2 GSH1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1497BioGRID
536187

Curated By

  • BioGRID