An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPII.

Jordan JD, He JC, Eungdamrong NJ, Gomes I, Ali W, Nguyen T, Bivona TG, Philips MR, Devi LA, Iyengar R

The G(alpha)o/i-coupled CB1 cannabionoid receptor induces neurite outgrowth in Neuro-2A cells. The mechanisms of signaling through G(alpha)o/i to induce neurite outgrowth were studied. The expression of G(alpha)o/i reduces the stability of its direct interactor protein, Rap1GAPII, by targeting it for ubiquitination and proteasomal degradation. This results in the activation of Rap1. G(alpha)o/i-induced activation of endogenous Rap1 in Neuro-2A cells is ... [more]

J. Biol. Chem. Mar. 25, 2005; 280(12);11413-21 [Pubmed: 15657046]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RAP1GAP

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activator activity [TAS]GTPase activity [NAS]Rap GTPase activator activity [IDA]Ras GTPase binding [IPI]protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

CNR1