An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.

Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Wan C, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J

Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N- and C-terminal tags and multiple promoters), is compatible with Gateway cloning, and incorporates a reference peptide. Its major advantage is that ... [more]

Mol. Cell Proteomics May. 01, 2010; 9(5);811-23 [Pubmed: 20305087]

Throughput

Low Throughput

Ontology Terms

hek-293 cell (BTO:0000007)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SMARCD1 KPNA2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9919	BioGRID	3138541

Curated By

BioGRID

Interaction Summary

SMARCD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein complex scaffold [IDA]transcription coactivator activity [NAS]

Gene Ontology Cellular Component

KPNA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase binding [IPI]nuclear localization sequence binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]