BAIT

RPT2

mts2, SPBC4.07c
19S proteasome regulatory subunit Rpt2
GO Process (1)
GO Function (2)
GO Component (4)
Schizosaccharomyces pombe (972h)
PREY

UBP6

SPAC6G9.08
ubiquitin C-terminal hydrolase Ubp6
Schizosaccharomyces pombe (972h)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Widespread cotranslational formation of protein complexes.

Duncan CD, Mata J

Most cellular processes are conducted by multi-protein complexes. However, little is known about how these complexes are assembled. In particular, it is not known if they are formed while one or more members of the complexes are being translated (cotranslational assembly). We took a genomic approach to address this question, by systematically identifying mRNAs associated with specific proteins. In a ... [more]

PLoS Genet. Dec. 01, 2011; 7(12);e1002398 [Pubmed: 22144913]

Throughput

  • High Throughput

Additional Notes

  • interaction detected by RIp-chip

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBP6 RPT2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
UBP6 RPT2
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
247855

Curated By

  • BioGRID