BAIT
CENPA
CENP-A, CenH3
centromere protein A
GO Process (6)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DDX5
G17P1, HLR1, HUMP68, p68
DEAD (Asp-Glu-Ala-Asp) box helicase 5
GO Process (11)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- cell growth [NAS]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- mRNA splicing, via spliceosome [IC]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- positive regulation of intracellular estrogen receptor signaling pathway [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of alternative mRNA splicing, via spliceosome [IDA]
- regulation of androgen receptor signaling pathway [IMP]
- regulation of osteoblast differentiation [ISS]
- regulation of skeletal muscle cell differentiation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres.
The human histone H3 variant, CENP-A, replaces the conventional histone H3 in centromeric chromatin and, together with centromere-specific DNA-binding factors, directs the assembly of the kinetochore. We purified the prenucelosomal e-CENP-A complex. We found that HJURP, a member of the complex, was required for cell cycle specific targeting of CENP-A to centromeres. HJURP facilitated efficient deposition of CENP-A/H4 tetramers to ... [more]
Proc. Natl. Acad. Sci. U.S.A. Jan. 26, 2010; 107(4);1349-54 [Pubmed: 20080577]
Throughput
- Low Throughput
Curated By
- BioGRID