PREY

NPM1

B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GO Process (23)
GO Function (14)
GO Component (10)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres.

Shuaib M, Ouararhni K, Dimitrov S, Hamiche A

The human histone H3 variant, CENP-A, replaces the conventional histone H3 in centromeric chromatin and, together with centromere-specific DNA-binding factors, directs the assembly of the kinetochore. We purified the prenucelosomal e-CENP-A complex. We found that HJURP, a member of the complex, was required for cell cycle specific targeting of CENP-A to centromeres. HJURP facilitated efficient deposition of CENP-A/H4 tetramers to ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 26, 2010; 107(4);1349-54 [Pubmed: 20080577]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NPM1 HJURP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9757BioGRID
1180821
NPM1 HJURP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9179BioGRID
2256675
NPM1 HJURP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9458BioGRID
3056903

Curated By

  • BioGRID