BAIT

NPL3

MTR13, MTS1, NAB1, NOP3, mRNA-binding protein NPL3, L000001270, YDR432W

RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress

GO Process (6)

GO Function (4)

GO Component (2)

Gene Ontology Biological Process

mRNA export from nucleus [IGI]

mRNA splicing, via spliceosome [IGI, IMP]

negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled [IDA, IMP]

negative regulation of translation [IDA]

positive regulation of transcription elongation from RNA polymerase II promoter [IDA, IMP]

translational termination [IGI, IMP]

Gene Ontology Molecular Function

RNA polymerase II core binding [IPI]
eukaryotic initiation factor 4G binding [IDA]
mRNA binding [IDA]
poly(A) binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IMP]

nucleus [IDA, IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RDN37-2

RDN37

35S ribosomal RNA (35S rRNA) transcript; encoded by the RDN1 locus; processed into the 25S, 18S and 5.8S rRNAs (represented by the RDN25, RDN18, and RDN58 loci)

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits.

Hackmann A, Gross T, Baierlein C, Krebber H

The nuclear export of large ribonucleoparticles is complex and requires specific transport factors. Messenger RNAs are exported through the RNA-binding protein Npl3 and the interacting export receptor Mex67. Export of large ribosomal subunits also requires Mex67; however, in this case, Mex67 binds directly to the 5S ribosomal RNA (rRNA) and does not require the Npl3 adaptor. Here, we have discovered ... [more]

EMBO Rep. Oct. 01, 2011; 12(10);1024-31 [Pubmed: 21852791]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary