BAIT

SLF1

SRO99, L000004191, L000002894, YDR515W
RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

YNL067W-B

YNL067W-A
Putative protein of unknown function
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

La-motif-dependent mRNA association with Slf1 promotes copper detoxification in yeast.

Schenk L, Meinel DM, Straesser K, Gerber AP

The La-motif (LAM) is an ancient and ubiquitous RNA-binding domain defining a superfamily of proteins, which comprises the genuine La proteins and La-related proteins (LARPs). In contrast to La, which binds and stabilizes pre-tRNAs and other RNA polymerase III transcripts, data on function and RNA targets of the LARPs have remained scarce. We have undertaken a global approach to elucidate ... [more]

RNA Mar. 01, 2012; 18(3);449-61 [Pubmed: 22271760]

Quantitative Score

  • 1.656666667 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • RNA-binding protein immunopurification-microarray (RIP-chip) analysis, cut-off score: >0.6

Curated By

  • BioGRID