PREY

GTF2E2

34kDa, AI462509, C330006J08Rik
general transcription factor II E, polypeptide 2 (beta subunit)
GO Process (0)
GO Function (2)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A murine ATFa-associated factor with transcriptional repressing activity.

De Graeve F, Bahr A, Chatton B, Kedinger C

The ATFa proteins, which are members of the CREB/ATF family of transcription factors, have previously been shown to interact with the adenovirus E1a oncoprotein and to mediate its transcriptional activity; they heterodimerize with Jun, Fos or related transcription factors, possibly altering their DNA-binding specificity; they also stably bind JNK2, a stress-induced protein kinase. Here we report the identification and characterization ... [more]

Oncogene Mar. 30, 2000; 19(14);1807-19 [Pubmed: 10777215]

Throughput

  • Low Throughput

Curated By

  • BioGRID