Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.

Nakayama M, Kikuno R, Ohara O

Large proteins have multiple domains that are potentially capable of binding many kinds of partners. It is conceivable, therefore, that such proteins could function as an intricate framework of assembly protein complexes. To comprehensively study protein-protein interactions between large KIAA proteins, we have constructed a library composed of 1087 KIAA cDNA clones based on prior functional classifications done in silico. ... [more]

Genome Res. Nov. 01, 2002; 12(11);1773-84 [Pubmed: 12421765]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KIAA1549

MYO5B

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IPI]microfilament motor activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

Two-hybrid

Publication

Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.

Throughput

Curated By

Rab GTPase binding [IPI]
microfilament motor activity [TAS]
protein binding [IPI]