PREY

MUSK

Mdk4, Mlk, Nsk1, Nsk2, Nsk3, RP23-170A3.1
muscle, skeletal, receptor tyrosine kinase
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A putative ariadne-like E3 ubiquitin ligase (PAUL) that interacts with the muscle-specific kinase (MuSK).

Bromann PA, Weiner JA, Apel ED, Lewis RM, Sanes JR

Formation of the postsynaptic membrane at the skeletal neuromuscular junction (NMJ) requires activation of the muscle-specific receptor tyrosine kinase (MuSK). Few intracellular mediators or modulators of MuSK actions are known. E3 ubiquitin ligases may serve this role, because activities of several receptor tyrosine kinases, G-protein-coupled receptors and channels are modulated by ubiquitination. Here, we report identification of a putative Ariadne-like ... [more]

Gene Expr. Patterns Jan. 01, 2004; 4(1);77-84 [Pubmed: 14678832]

Throughput

  • Low Throughput

Curated By

  • BioGRID