An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rapid Ca2+-dependent decrease of protein ubiquitination at synapses.

Chen H, Polo S, Di Fiore PP, De Camilli PV

Protein ubiquitination has been implicated in the regulation of axonal growth and synaptic plasticity as well as in the pathogenesis of neurodegenerative diseases. Here we show that depolarization-dependent Ca2+ influx into synaptosomes produces a global, rapid (range of seconds), and reversible decrease of the ubiquitinated state of proteins, which correlates with the Ca2+-dependent dephosphorylation of several synaptic proteins. A similar ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 09, 2003; 100(25);14908-13 [Pubmed: 14657369]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBC EPN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Na CH (2012)	Low	-	BioGRID	-
EPN1 UBC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Sato Y (2009)	Low	-	BioGRID	685052

Curated By

BioGRID

Interaction Summary

EPN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

transcription factor binding [IPI]

Gene Ontology Cellular Component

UBC