LYN
Gene Ontology Biological Process
- B cell homeostasis [ISO, ISS]
- B cell receptor signaling pathway [ISO]
- Fc receptor mediated inhibitory signaling pathway [ISO, ISS]
- Fc receptor mediated stimulatory signaling pathway [ISO, ISS]
- cell migration [IBA]
- cellular response to DNA damage stimulus [IBA, ISO]
- cellular response to extracellular stimulus [IEP]
- cellular response to heat [IEP]
- cellular response to peptide hormone stimulus [IBA]
- cellular response to retinoic acid [ISO]
- chemotaxis [IBA]
- cytokine secretion [IMP]
- dendritic cell differentiation [IBA, ISO, ISS]
- erythrocyte differentiation [ISO, ISS]
- hemopoiesis [ISO]
- histamine secretion by mast cell [IMP]
- immune response-regulating cell surface receptor signaling pathway [ISO, ISS]
- innate immune response [IBA]
- intracellular signal transduction [ISO]
- lipopolysaccharide-mediated signaling pathway [ISO, ISS]
- negative regulation of B cell proliferation [IBA, ISO]
- negative regulation of ERK1 and ERK2 cascade [ISO, ISS]
- negative regulation of MAP kinase activity [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of intracellular signal transduction [ISO, ISS]
- negative regulation of mast cell proliferation [IBA, ISO, ISS]
- negative regulation of myeloid leukocyte differentiation [ISO]
- negative regulation of protein phosphorylation [ISO, ISS]
- negative regulation of toll-like receptor 2 signaling pathway [ISO, ISS]
- negative regulation of toll-like receptor 4 signaling pathway [ISO, ISS]
- neuron projection development [IBA, ISO]
- oligodendrocyte development [IEP]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [ISO]
- platelet degranulation [IBA, ISO, ISS]
- positive regulation of B cell receptor signaling pathway [ISO]
- positive regulation of Fc receptor mediated stimulatory signaling pathway [IMP]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [ISO, ISS]
- positive regulation of cellular component movement [ISO]
- positive regulation of dendritic cell apoptotic process [IBA, ISO, ISS]
- positive regulation of glial cell proliferation [IDA]
- positive regulation of mast cell proliferation [ISO]
- positive regulation of neuron projection development [ISO]
- positive regulation of oligodendrocyte progenitor proliferation [IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [ISO]
- positive regulation of phosphorylation [IMP]
- positive regulation of stress-activated protein kinase signaling cascade [ISO]
- positive regulation of tyrosine phosphorylation of STAT protein [ISO, ISS]
- protein autophosphorylation [ISO]
- protein phosphorylation [ISO]
- regulation of B cell apoptotic process [IBA, ISO]
- regulation of B cell receptor signaling pathway [IBA, ISO, ISS]
- regulation of ERK1 and ERK2 cascade [ISO, ISS]
- regulation of cell adhesion mediated by integrin [ISO]
- regulation of cytokine production [ISO, ISS]
- regulation of cytokine secretion [ISO]
- regulation of erythrocyte differentiation [ISO, ISS]
- regulation of inflammatory response [ISO]
- regulation of mast cell activation [ISO, ISS]
- regulation of mast cell degranulation [IBA, ISO, ISS]
- regulation of monocyte chemotaxis [ISO]
- regulation of platelet aggregation [IBA, ISO, ISS]
- regulation of release of sequestered calcium ion into cytosol [IMP, ISO]
- response to amino acid [IEP]
- response to axon injury [IEP]
- response to carbohydrate [IEP]
- response to drug [IEP]
- response to hormone [ISO, ISS]
- response to insulin [IEP]
- response to organic cyclic compound [IEP]
- response to peptide hormone [IEP]
- response to sterol depletion [IEP]
- response to toxic substance [IEP]
- tolerance induction to self antigen [IBA, ISO, ISS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA, ISO]
Gene Ontology Molecular Function- SH3 domain binding [ISO]
- enzyme binding [IPI, ISO]
- gamma-tubulin binding [IDA]
- glycosphingolipid binding [IPI]
- integrin binding [IDA]
- ion channel binding [ISO]
- kinase activity [TAS]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI, ISO]
- protein kinase activity [ISO]
- protein tyrosine kinase activity [IDA, ISO]
- receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- SH3 domain binding [ISO]
- enzyme binding [IPI, ISO]
- gamma-tubulin binding [IDA]
- glycosphingolipid binding [IPI]
- integrin binding [IDA]
- ion channel binding [ISO]
- kinase activity [TAS]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI, ISO]
- protein kinase activity [ISO]
- protein tyrosine kinase activity [IDA, ISO]
- receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- cell [ISO]
- cell-cell adherens junction [IDA]
- cytoplasm [ISO, ISS]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IBA]
- integrin alpha2-beta1 complex [IDA]
- mast cell granule [IMP]
- membrane [IDA]
- membrane raft [IDA, ISO]
- mitochondrial crista [IDA]
- mitochondrial intermembrane space [IDA]
- mitochondrial membrane [IDA]
- nucleus [ISO, ISS]
- perinuclear region of cytoplasm [IDA, ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA]
UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Negative regulation of Lyn protein-tyrosine kinase by c-Cbl ubiquitin-protein ligase in Fc epsilon RI-mediated mast cell activation.
Recent studies have demonstrated that c-Cbl functions as a ubiquitin-protein ligase toward immune receptors and non-receptor protein-tyrosine kinase Syk by facilitating their ubiquitination and subsequent targeting to proteasomes. However, it was not clear whether Src family kinase Lyn is regulated by the Cbl family of ubiquitin-protein ligases.Aggregation of the high affinity IgE receptor (Fc epsilon RI) induces the rapid ubiquitination ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBC LYN | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| LYN UBC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| LYN UBC | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID