BAIT
HSPD1
Hsp60, Hspd1-30p
heat shock protein 1 (chaperonin)
GO Process (35)
GO Function (12)
GO Component (23)
Gene Ontology Biological Process
- 'de novo' protein folding [IC]
- B cell activation [ISO]
- B cell cytokine production [ISO]
- B cell proliferation [ISO]
- MyD88-dependent toll-like receptor signaling pathway [ISO]
- T cell activation [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- chaperone mediated protein folding requiring cofactor [IDA]
- detection of misfolded protein [IDA]
- isotype switching to IgG isotypes [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of neuron apoptotic process [IMP]
- positive regulation of T cell activation [ISO]
- positive regulation of T cell mediated immune response to tumor cell [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of inflammatory response [IDA]
- positive regulation of interferon-alpha production [ISO]
- positive regulation of interferon-gamma production [ISO]
- positive regulation of interleukin-10 production [ISO]
- positive regulation of interleukin-12 production [ISO]
- positive regulation of interleukin-6 production [ISO]
- positive regulation of macrophage activation [ISO]
- protein refolding [ISO]
- protein stabilization [ISO]
- response to ATP [IEP]
- response to cocaine [IEP]
- response to drug [IEP]
- response to estrogen [IEP]
- response to heat [IEP]
- response to hydrogen peroxide [IEP]
- response to hypoxia [IEP]
- response to lipopolysaccharide [IEP]
- response to organic cyclic compound [IEP]
- response to organic substance [IEP]
- response to unfolded protein [ISO]
Gene Ontology Molecular Function- chaperone binding [ISO]
- double-stranded RNA binding [ISO]
- insulin binding [IPI]
- lipopolysaccharide binding [ISO]
- misfolded protein binding [IPI]
- p53 binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein heterodimerization activity [IPI]
- ubiquitin protein ligase binding [ISO]
- chaperone binding [ISO]
- double-stranded RNA binding [ISO]
- insulin binding [IPI]
- lipopolysaccharide binding [ISO]
- misfolded protein binding [IPI]
- p53 binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein heterodimerization activity [IPI]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell surface [IDA, ISO]
- coated pit [ISO]
- coated vesicle [ISO]
- cyclin-dependent protein kinase activating kinase holoenzyme complex [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- early endosome [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipopolysaccharide receptor complex [ISO]
- membrane [ISO]
- membrane raft [IDA]
- mitochondrial crista [IDA]
- mitochondrial inner membrane [ISO]
- mitochondrion [IDA, ISO]
- myelin sheath [ISO]
- plasma membrane [ISO]
- protein complex [ISO, ISS]
- rough endoplasmic reticulum [IDA]
- secretory granule [IDA, ISO]
- zymogen granule [IDA]
Rattus norvegicus
PREY
BCL2L1
Bcl-xl, Bcl2l, Bclx, bcl-X
Bcl2-like 1
GO Process (50)
GO Function (9)
GO Component (11)
Gene Ontology Biological Process
- apoptotic process [IEP]
- apoptotic process in bone marrow [ISO]
- cell proliferation [ISO]
- cellular process regulating host cell cycle in response to virus [ISO]
- cellular response to alkaloid [ISO]
- cellular response to amino acid stimulus [ISO]
- cellular response to gamma radiation [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA, ISO]
- fertilization [ISO]
- germ cell development [ISO]
- growth [ISO]
- hepatocyte apoptotic process [ISO]
- in utero embryonic development [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA, ISO]
- male gonad development [ISO]
- mitochondrion morphogenesis [ISO]
- mitotic cell cycle checkpoint [ISO, ISS]
- negative regulation of anoikis [ISO]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of establishment of protein localization to plasma membrane [ISO]
- negative regulation of execution phase of apoptosis [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [IBA, ISO]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- neuron apoptotic process [ISO]
- ovarian follicle development [IMP, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell proliferation [ISO]
- regulation of apoptotic process [IDA]
- regulation of mitochondrial membrane permeability [ISO]
- regulation of mitochondrial membrane potential [ISO]
- release of cytochrome c from mitochondria [ISO]
- response to cycloheximide [ISO]
- response to cytokine [ISO]
- response to electrical stimulus [IEP]
- response to hydrogen peroxide [IEP]
- response to hypoxia [IEP]
- response to inorganic substance [IEP]
- response to ischemia [IDA]
- response to lead ion [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IEP]
- response to oxidative stress [IEP]
- response to peptide hormone [IEP]
- response to radiation [ISO]
- response to virus [ISO]
- spermatogenesis [ISO]
- suppression by virus of host apoptotic process [ISO]
Gene Ontology Molecular Function- BH domain binding [IPI]
- BH3 domain binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- identical protein binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA]
- protein kinase binding [ISO]
- BH domain binding [IPI]
- BH3 domain binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IDA]
- identical protein binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IBA]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Hsp10 and Hsp60 modulate Bcl-2 family and mitochondria apoptosis signaling induced by doxorubicin in cardiac muscle cells.
The development of doxorubicin cardiomyopathy involves apoptosis of cardiac muscle cells. This study was carried out to define the roles of two heat-shock proteins, Hsp10 and Hsp60, on doxorubicin-induced apoptosis in primary cardiomyocytes. Doxorubicin induces apoptosis of cardiomyocytes by activating mitochondria apoptosis signaling. Transducing cardiomyocytes with Hsp10 or Hsp60 with adenoviral vector suppressed the occurrence of apoptosis in the doxorubicin-treated ... [more]
J. Mol. Cell. Cardiol. Sep. 01, 2003; 35(9);1135-43 [Pubmed: 12967636]
Throughput
- Low Throughput
Curated By
- BioGRID