BAIT
NCB2
YDR1, L000003241, YDR397C
Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p
GO Process (6)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IMP]
- negative regulation of tRNA transcription from RNA polymerase III promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]
- regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP]
Gene Ontology Molecular Function- TBP-class protein binding RNA polymerase II transcription factor activity [IDA, IPI]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IMP]
- chromatin binding [IDA]
- core promoter binding [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [IDA, IGI, IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity [IDA, IPI]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IMP]
- chromatin binding [IDA]
- core promoter binding [IDA]
- transcription coactivator activity [IDA]
- transcription corepressor activity [IDA, IGI, IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MED2
L000003914, YDL005C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia
GO Process (3)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Specific defects in different transcription complexes compensate for the requirement of the negative cofactor 2 repressor in Saccharomyces cerevisiae.
Negative cofactor 2 (NC2) has been described as an essential and evolutionarily conserved transcriptional repressor, although in vitro and in vivo experiments suggest that it can function as both a positive and a negative effector of transcription. NC2 operates by interacting with the core promoter and components of the basal transcription machinery, like the TATA-binding protein (TBP). In this work, ... [more]
Genetics May. 01, 2007; 176(1);125-38 [Pubmed: 17339209]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID