BAIT
CHD5
4930532L22Rik, AW060752, B230399N07Rik, CHD-5, RP23-421E12.10-001
chromodomain helicase DNA binding protein 5
GO Process (9)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- cerebral cortex neuron differentiation [IMP]
- histone H3-K27 trimethylation [ISO]
- histone H4 acetylation [IMP]
- negative regulation of cell proliferation [IMP]
- positive regulation of signal transduction by p53 class mediator [IMP]
- regulation of cell differentiation [IMP]
- regulation of transcription involved in cell fate commitment [IMP, ISO]
- spermatogenesis, exchange of chromosomal proteins [IMP]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function
Mus musculus
PREY
HDAC2
D10Wsu179e, YAF1, Yy1bp, mRPD3
histone deacetylase 2
GO Process (43)
GO Function (18)
GO Component (13)
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [ISO]
- cardiac muscle cell development [IMP]
- cellular response to hydrogen peroxide [ISO]
- chromatin modification [TAS]
- circadian regulation of gene expression [IDA]
- dendrite development [IMP]
- embryonic digit morphogenesis [IGI]
- epidermal cell differentiation [IGI]
- eyelid development in camera-type eye [IGI]
- fungiform papilla formation [IGI]
- hair follicle placode formation [IGI]
- hippocampus development [IGI]
- histone H3 deacetylation [IDA, ISO]
- histone H4 deacetylation [IDA]
- histone deacetylation [IGI, ISO, TAS]
- maintenance of chromatin silencing [ISO]
- negative regulation of DNA binding [ISO]
- negative regulation of apoptotic process [IGI]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cardiac muscle cell proliferation [IMP]
- negative regulation of dendritic spine development [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [IGI]
- negative regulation of neuron projection development [IDA, ISO]
- negative regulation of peptidyl-lysine acetylation [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IGI, ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- neuron differentiation [IGI]
- odontogenesis of dentin-containing tooth [IGI]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of interleukin-1 production [ISO]
- positive regulation of oligodendrocyte differentiation [IGI, ISO]
- positive regulation of proteolysis [ISO]
- positive regulation of receptor biosynthetic process [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- positive regulation of tyrosine phosphorylation of Stat3 protein [ISO]
- protein deacetylation [IDA]
- regulation of protein deacetylation [IGI]
- regulation of protein kinase B signaling [IMP]
- regulation of sarcomere organization [IMP]
Gene Ontology Molecular Function- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [ISO]
- deacetylase activity [IDA]
- enzyme binding [IPI, ISO]
- heat shock protein binding [ISO]
- histone deacetylase activity [IDA, ISO, TAS]
- nucleosomal DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein deacetylase activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO, TAS]
- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [ISO]
- deacetylase activity [IDA]
- enzyme binding [IPI, ISO]
- heat shock protein binding [ISO]
- histone deacetylase activity [IDA, ISO, TAS]
- nucleosomal DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein deacetylase activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO, TAS]
Gene Ontology Cellular Component
- ESC/E(Z) complex [ISO]
- NuRD complex [ISO]
- Sin3 complex [ISO]
- chromatin [ISO]
- cytoplasm [ISO, TAS]
- heterochromatin [IDA]
- histone deacetylase complex [TAS]
- nuclear chromatin [IDA, ISO]
- nucleus [IDA, ISO]
- protein complex [ISO]
- replication fork [IDA]
- transcription factor complex [IPI]
- transcriptional repressor complex [IPI]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CHD5, a brain-specific paralog of Mi2 chromatin remodeling enzymes, regulates expression of neuronal genes.
CHD5 is frequently deleted in neuroblastoma and is a tumor suppressor gene. However, little is known about the role of CHD5 other than it is homologous to chromatin remodeling ATPases. We found CHD5 mRNA was restricted to the brain; by contrast, most remodeling ATPases were broadly expressed. CHD5 protein isolated from mouse brain was associated with HDAC2, p66ss, MTA3 and ... [more]
PLoS ONE Sep. 21, 2011; 6(9);e24515 [Pubmed: 21931736]
Throughput
- Low Throughput
Curated By
- BioGRID