SKP2
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- G2/M transition of mitotic cell cycle [IGI, IMP]
- positive regulation of intracellular estrogen receptor signaling pathway [IGI]
- positive regulation of protein polyubiquitination [IDA]
- positive regulation of smooth muscle cell proliferation [ISO]
- protein polyubiquitination [IDA]
- regulation of apoptotic process [ISO]
- regulation of cell cycle [IPI]
- ubiquitin-dependent protein catabolic process [IDA, IMP]
Gene Ontology Molecular Function
CDKN1B
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- autophagic cell death [ISO]
- cell cycle arrest [IDA, ISO]
- cell death [ISO]
- cellular response to antibiotic [IDA]
- cellular response to lithium ion [ISO]
- cellular response to organic cyclic compound [IDA]
- inner ear development [IMP]
- mitotic cell cycle arrest [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cellular component movement [IDA]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of epithelial cell proliferation [IMP]
- negative regulation of epithelial cell proliferation involved in prostate gland development [IMP]
- negative regulation of kinase activity [ISO]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of phosphorylation [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of cell death [ISO]
- positive regulation of cell proliferation [IMP]
- positive regulation of microtubule polymerization [IGI]
- potassium ion transport [IGI]
- sensory perception of sound [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A CDK-independent function of mammalian Cks1: targeting of SCF(Skp2) to the CDK inhibitor p27Kip1.
The Cks/Suc1 proteins associate with CDK/cyclin complexes, but their precise function(s) is not well defined. Here we demonstrate that Cks1 directs the ubiquitin-mediated proteolysis of the CDK-bound substrate p27Kip1 by the protein ubiquitin ligase (E3) SCF(Skp2). Cks1 associates with the F box protein Skp2 and is essential for recognition of the p27Kip1 substrate for ubiquitination in vivo and in vitro. ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID