APOBEC3G
Gene Ontology Biological Process
- DNA cytosine deamination [IDA]
- base conversion or substitution editing [TAS]
- cytidine deamination [IDA]
- defense response to virus [IDA]
- innate immune response [IDA]
- negative regulation of single stranded viral RNA replication via double stranded DNA intermediate [IDA]
- negative regulation of transposition [IDA]
- negative regulation of viral genome replication [IDA]
- negative regulation of viral process [IDA]
- positive regulation of defense response to virus by host [IDA]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
APOBEC3G
Gene Ontology Biological Process
- DNA cytosine deamination [IDA]
- base conversion or substitution editing [TAS]
- cytidine deamination [IDA]
- defense response to virus [IDA]
- innate immune response [IDA]
- negative regulation of single stranded viral RNA replication via double stranded DNA intermediate [IDA]
- negative regulation of transposition [IDA]
- negative regulation of viral genome replication [IDA]
- negative regulation of viral process [IDA]
- positive regulation of defense response to virus by host [IDA]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
HIV-1 Vif interaction with APOBEC3 deaminases and its characterization by a new sensitive assay.
The human APOBEC3 (A3) cytidine deaminases, such as APOBEC3G (A3G) and APOBEC3F (A3F), are potent inhibitors of Vif-deficient human immunodeficiency virus type 1 (HIV-1). HIV-1 Vif (viral infectivity factor) binds A3 proteins and targets these proteins for ubiquitination and proteasomal degradation. As such, the therapeutic blockage of Vif-A3 interaction is predicted to stimulate natural antiviral activity by rescuing APOBEC expression ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| APOBEC3G APOBEC3G | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID