CHEK2
Gene Ontology Biological Process
- DNA damage checkpoint [TAS]
- DNA damage induced protein phosphorylation [IMP]
- G2/M transition of mitotic cell cycle [IMP]
- cellular protein catabolic process [IMP]
- cellular response to DNA damage stimulus [IMP, TAS]
- double-strand break repair [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- protein stabilization [IDA]
- regulation of protein catabolic process [IMP]
- regulation of transcription, DNA-templated [IDA]
- replicative senescence [NAS]
- signal transduction in response to DNA damage [IDA]
- signal transduction involved in intra-S DNA damage checkpoint [IMP]
- spindle assembly involved in mitosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPP2R5D
Gene Ontology Biological Process
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- carbohydrate metabolic process [TAS]
- dephosphorylation [TAS]
- energy reserve metabolic process [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- innate immune response [TAS]
- nervous system development [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of catalytic activity [TAS]
- small molecule metabolic process [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Regulation of Chk2 phosphorylation by interaction with protein phosphatase 2A via its B' regulatory subunit.
Chk2 is a key player of the DNA damage signalling pathway. To identify new regulators of this kinase, we performed a yeast two-hybrid screen and found that Chk2 associated with the B' regulatory subunit of protein phosphatase PP2A. In vitro GST-Chk2 pulldowns demonstrated that B'gamma isoforms bound to Chk2 with the strongest apparent affinity. This was confirmed in cellulo by ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CHEK2 PPP2R5D | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID