BAIT

PP1-87B

87B, CG5650, DmPp1-87B, Dmel\CG5650, PP-1alpha, PP1, PP1 87B, PP1 beta87b, PP187B, PP1A87B, PP1B, PP1alpha-87B, PP1alpha87B, PP1alpha[[2]], PP1c, PP1c 87B, PPI 87B, Pp1-87Bb, Ppl-87B, Su(Var)3-6, Su(var)(3)6, Su-var(3)6, Suvar(3)6, ck19, dPP1beta, l(3)87Bg, l(3)ck19, l(3)j6E7, Dmel_CG5650
Protein phosphatase 1 at 87B
Drosophila melanogaster
PREY

I-2

CG10574, DI-2, Dm I-2, Dmel\CG10574, I-2Dm, I-2PP1, SET, Dmel_CG10574
Inhibitor-2
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein complex network of Drosophila melanogaster.

Guruharsha KG, Rual JF, Zhai B, Mintseris J, Vaidya P, Vaidya N, Beekman C, Wong C, Rhee DY, Cenaj O, McKillip E, Shah S, Stapleton M, Wan KH, Yu C, Parsa B, Carlson JW, Chen X, Kapadia B, VijayRaghavan K, Gygi SP, Celniker SE, Obar RA, Artavanis-Tsakonas S

Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a ... [more]

Cell Oct. 28, 2011; 147(3);690-703 [Pubmed: 22036573]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PP1-87B I-2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-FlyBase
-
I-2 PP1-87B
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID