ROT1
Gene Ontology Biological Process
- 'de novo' protein folding [IMP, IPI]
- budding cell apical bud growth [IGI, IMP]
- endoplasmic reticulum unfolded protein response [IGI]
- establishment or maintenance of actin cytoskeleton polarity [IMP]
- fungal-type cell wall biogenesis [IMP]
- protein N-linked glycosylation [IGI, IPI]
- protein O-linked mannosylation [IGI]
- protein folding [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
OST3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Enhancement
A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast.
Rot1 is an essential yeast protein originally shown to be implicated in such diverse processes such as β-1,6-glucan synthesis, actin cytoskeleton dynamics, or lysis of autophagic bodies. More recently also a role as a molecular chaperone has been discovered. Here we report that Rot1 interacts in a synthetic manner with Ost3, one of the nine subunits of the oligosaccharyltransferase complex, ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: phenotypic enhancement (APO:0000177) [phenotypic enhancement (APO:0000177)]
Additional Notes
- double mutant has no growth when stressed by agents known to interfere with cell wall biogenesis, such as Calcofluor White, caffeine, Congo Red or hygromycin B. figure 1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ROT1 OST3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 656175 | |
| OST3 ROT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2339 | BioGRID | 2070023 | |
| ROT1 OST3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | - | BioGRID | 210821 | |
| OST3 ROT1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -4.9134 | BioGRID | 209831 | |
| ROT1 OST3 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 656173 |
Curated By
- BioGRID