BAIT
RNH1
L000001653, YMR234W
Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
REV7
L000001618, YIL139C
Accessory subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev3p, Pol31p and Pol32p
GO Process (2)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
RNase H and postreplication repair protect cells from ribonucleotides incorporated in DNA.
The chemical identity and integrity of the genome is challenged by the incorporation of ribonucleoside triphosphates (rNTPs) in place of deoxyribonucleoside triphosphates (dNTPs) during replication. Misincorporation is limited by the selectivity of DNA replicases. We show that accumulation of ribonucleoside monophosphates (rNMPs) in the genome causes replication stress and has toxic consequences, particularly in the absence of RNase H1 and ... [more]
Mol. Cell Jan. 13, 2012; 45(1);99-110 [Pubmed: 22244334]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- deletion of mms2 and all components of translesion DNA synthesis (rev1, rev7,rev3 and rad30) in an rnh1/rnh201 mutant background is lethal in the presence of HU (CID 3657 CHEBI 44423)
- genetic complex
Curated By
- BioGRID