UBA1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBD
Gene Ontology Biological Process
- aggresome assembly [IDA]
- myeloid dendritic cell differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- positive regulation of apoptotic process [IMP]
- protein modification by small protein conjugation [NAS]
- protein ubiquitination [IDA]
- proteolysis [NAS]
- response to interferon-gamma [IEP]
- response to tumor necrosis factor [IEP]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis.
FAT10 is the only ubiquitin-like modifier that can target proteins for degradation by the proteasome in a ubiquitin-independent manner. The degradation of FAT10-linked proteins by the proteasome is strongly accelerated by the ubiquitin-like-ubiquitin-associated protein NEDD8 ultimate buster-1 long (NUB1L). Here we show how FAT10 and NUB1L dock with the 26S proteasome to initiate proteolysis. We identify the 26S proteasome subunit ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID