MET31
Gene Ontology Biological Process
Gene Ontology Molecular Function
MET32
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of mitotic cell cycle [IGI, IMP]
- regulation of sulfur amino acid metabolic process [IDA, IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, IGI]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Characterizing roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.
Yeast sulfur metabolism is transcriptionally regulated by the activator Met4. Met4 lacks DNA-binding ability and relies on interactions with Met31 and Met32, paralogous proteins that bind the same cis-regulatory element, to activate its targets. While Met31 and Met32 are redundant for growth in the absence of methionine, studies indicate that Met32 has a prominent role over Met31 when Met30, a ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: utilization of sulfur source (APO:0000219)
Additional Notes
- double mutants show increased defects in utilization of common sulfur sources and sulfonates
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MET31 MET32 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -11.6277 | BioGRID | 541312 | |
| MET31 MET32 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.8827 | BioGRID | 420894 | |
| MET32 MET31 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.8827 | BioGRID | 368579 | |
| MET32 MET31 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.6334 | BioGRID | 2098264 | |
| MET31 MET32 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.8446 | BioGRID | 2189261 | |
| MET32 MET31 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -13.9695 | BioGRID | 508977 | |
| MET31 MET32 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 156373 | |
| MET31 MET32 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 435859 | |
| MET31 MET32 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 2890067 | |
| MET31 MET32 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 163866 |
Curated By
- BioGRID