BAIT

SLI15

L000003320, YBR156C
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

BIM1

YEB1, microtubule-binding protein BIM1, EB1, L000003272, YER016W
Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Spatiotemporal regulation of ipl1/aurora activity by direct cdk1 phosphorylation.

Zimniak T, Fitz V, Zhou H, Lampert F, Opravil S, Mechtler K, Stolt-Bergner P, Westermann S

Oscillating cyclin-dependent kinase 1 (Cdk1) activity is the major regulator of cell-cycle progression [1], whereas the Aurora B kinase, as part of the chromosome passenger complex (CPC), controls critical aspects of mitosis such as chromosome condensation and biorientation on the spindle [2]. How these kinases mechanistically coordinate their important functions is only partially understood. Here, using budding yeast, we identify ... [more]

Curr. Biol. May. 08, 2012; 22(9);787-93 [Pubmed: 22521784]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112) [synthetic lethality (APO:0000183)]

Additional Notes

  • figure S3C.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BIM1 SLI15
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
BIM1 SLI15
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.471BioGRID
2039328
BIM1 SLI15
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low/High-BioGRID
438940
BIM1 SLI15
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
SLI15 BIM1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID