BAIT

KHA1

YJL094C
Putative K+/H+ antiporter; has a probable role in intracellular cation homeostasis; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

YPT6

Rab family GTPase YPT6, L000002896, L000002948, YLR262C
Rab family GTPase; Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; resides temporarily at the Golgi, dissociates into cytosol upon arrival of the Rab GTPaseYpt32p, which also functions in the late Golgi; Golgi-localized form is bound to GTP, while cytosolic form is GDP-bound; homolog of the mammalian Rab6
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Comparison of the influence of small GTPases Arl1 and Ypt6 on yeast cells' tolerance to various stress factors.

Maresova L, Vydareny T, Sychrova H

The GTPases Arl1 and Ypt6 are involved in the intracellular transport of vesicles and their fusion with the trans-Golgi network. This work is focused on comparing the roles of these GTPases in the tolerance of S. cerevisiae cells to an increased concentration of alkali metal cations and other stress factors. We studied the phenotypes of arl1 or ypt6 deletions in ... [more]

Unknown Dec. 22, 2011; 0(0); [Pubmed: 22188384]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • kha1 ypt6 double mutant (in nha1 ena1 ena2 ena3 ena4 background) has increased sensitivity to high pH
  • kha1 ypt6 double mutant (in nha1 ena1 ena2 ena3 ena4 nhx1 background) has increased sensitivity to hygromycin B

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YPT6 KHA1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.5466BioGRID
583520

Curated By

  • BioGRID