Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of multi-SH3 domain-containing protein interactome in pancreatic cancer: a yeast two-hybrid approach.

Thalappilly S, Suliman M, Gayet O, Soubeyran P, Hermant A, Lecine P, Iovanna JL, Dusetti NJ

Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease that shows minimal response to chemotherapy. Genetic changes involved in the progression of PDAC concern genes that encode proteins related to signal transduction networks. This fact reveals the importance in identifying the role and the relations between multiple signaling cascades in PDAC. One of the major factors that modulate signaling events is ... [more]

Proteomics Aug. 01, 2008; 8(15);3071-81 [Pubmed: 18654987]

Throughput

  • Low Throughput

Curated By

  • BioGRID