KIF3A
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- anterior/posterior pattern specification [IMP]
- cilium assembly [IMP]
- cilium morphogenesis [IMP]
- determination of left/right symmetry [IMP]
- dorsal/ventral neural tube patterning [IGI, IMP]
- dorsal/ventral pattern formation [IMP]
- epidermal stem cell homeostasis [IMP]
- epidermis development [IMP]
- heart development [IMP]
- in utero embryonic development [IGI]
- inner ear receptor stereocilium organization [IMP]
- kidney development [IMP]
- microtubule-based movement [IBA]
- positive regulation of epithelial cell proliferation [IMP]
- positive regulation of receptor-mediated endocytosis [ISO]
- smoothened signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axoneme [ISO]
- centrosome [ISO]
- cilium [IDA]
- extracellular vesicular exosome [ISO]
- intraciliary transport particle [IDA]
- kinesin II complex [IDA, ISO]
- microtubule cytoskeleton [ISO]
- neuron projection [IDA]
- photoreceptor connecting cilium [IDA]
- primary cilium [IDA]
- spindle microtubule [ISO]
- synaptic vesicle [ISO]
TNKS
Gene Ontology Biological Process
- negative regulation of DNA binding [ISO]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of telomere maintenance via telomerase [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein ADP-ribosylation [ISO]
- protein auto-ADP-ribosylation [ISO]
- protein localization to chromosome, telomeric region [ISO]
- protein poly-ADP-ribosylation [ISO]
- protein polyubiquitination [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The Axin/TNKS complex interacts with KIF3A and is required for insulin-stimulated GLUT4 translocation.
Insulin-stimulated glucose uptake by the glucose transporter GLUT4 plays a central role in whole-body glucose homeostasis, dysregulation of which leads to type 2 diabetes. However, the molecular components and mechanisms regulating insulin-stimulated glucose uptake remain largely unclear. Here, we demonstrate that Axin interacts with the ADP-ribosylase tankyrase 2 (TNKS2) and the kinesin motor protein KIF3A, forming a ternary complex crucial ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TNKS KIF3A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID